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. 2012 Feb 14;7(2):e31691. doi: 10.1371/journal.pone.0031691

Figure 1. Collagen content, cross-linking, and mRNA expression in LD muscle of offspring sheep from Con (□) and OB (▪) ewes.

Figure 1

A: Collagen content calculated based on the hydroxylproline concentration showing an increase in collagen concentration in OB offspring LD muscle (% dry muscle weight). B and C: Representative images of LD muscle from Con and OB offspring sheep at different magnifications. Transverse section of LD muscle stained with Masson's trichrome showing muscle fibers (red) and connective tissue (blue). D: Collagen cross-linking was higher in OB offspring muscle based on the concentration of pyridinoline (nmole/mg of dry muscle weight). E: Type I and III collagen expression and type III/I ratio of LD muscle. (*P<0.05, & P<0.10; Mean ± SE; n = 7).