Skip to main content
. 2012 Feb 14;7(2):e31862. doi: 10.1371/journal.pone.0031862

Figure 4. MNR instabilities in hsa-mir-1273c (T11), hsa-mir-567 (A13) and hsa-mir-1303 (T13).

Figure 4

Allelic profiles for several MSI CRC cell lines and primary tumors are shown. Normal profiles are defined in LBL and MSS cell lines and primary tumors. For monomorphic genes, a dashed vertical line indicates the unique allele. The polymorphic zone for hsa-mir-1303 is defined between two dashed vertical lines going along the 2 alleles (see Figure S1). Sizes (bp) are indicated in a box below each profile. Various allelic deletions ranging from 1 to 4 bp were observed in MSI CRC cell lines and primary tumors and are indicated in bold. The observed deletions were sometimes bi-allelic in MSI CRC cell lines. In MSI primary tumors, the allelic profiles were also highly suggestive of bi-allelic mutations. Due to the inherent polymorphism that can modify the length of the sequence, the hairpin sequence of hsa-mir-1303 was determined for a correct and reliable evaluation of the alterations in MSI CRC cell lines (see Table S2).