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. 2010 Aug 3;59(10):2426–2434. doi: 10.2337/db09-1870

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© 2010 by the American Diabetes Association.

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FIG. 2. — Dose response and time course of the effects of leucine on AMPK phosphorylation and activity. EDL muscles were incubated in the absence (0) or presence (50, 100, or 200 μmol/l) of leucine for 1 h and muscle lysates analyzed for the phosphorylation of AMPK and its downstream target P-ACC using SDS-PAGE (A) and for α2-AMPK activity (B). C: The time course (15–120 min) of changes in the abundance of P-AMPK in muscles incubated with 100 μmol/l leucine. D: Comparison of effects of leucine, isoleucine, and glutamine (100 μmol/l) on total AMPK and P-AMPK. Results are means ± SE (n = 6). *P < 0.05 relative to 0 μmol/l leucine or time 0. (A high-quality color representation of this figure is available in the online issue.)