FIG. 5.

Effect of AICAR on protein synthesis; AMPK α1 and α2 activity and phosphorylation; mTOR, p70S6K, and ACC phosphorylation; and malonyl CoA content. EDL muscles were preincubated with Krebs-Henseleit solution containing 5.5 mmol/l glucose for 20 min and then for an additional 1 h in the presence of 1 mmol/l AICAR. Protein synthesis was measured at 0 or 5.5 mmol/l glucose or 5.5 mmol/l glucose and AICAR (1 mmol/l) (A). Muscle supernatants were subjected to immunopecipitation with specific antibodies to the α1 and α2 of AMPK (B). AMPK (B), ACC (C), and mTOR and p70S6K (D) phosphorylation were determined. Malonyl CoA concentrations (C) were determined as described in research design and methods. P-AMPK, P-ACC, P-mTOR, and P-p70S6K at 0 mmol/l glucose are shown in Figs. 3 and 4. Results are the means ± SE of three experiments. *P < 0.05 vs. 5.5 mmol/l glucose. *P < 0.01 vs. 5.5 mmol/l glucose. (A high-quality color representation of this figure is available in the online issue.)