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. 2010 Jul 9;59(10):2549–2557. doi: 10.2337/db10-0480

FIG. 5.

FIG. 5.

Increased inflammatory chemokine expression in Bcl3−/− mice. A: Total RNA was extracted from the pancreata and spleens of 6- to 8-week-old Bcl3+/+ and Bcl3−/− NOD mice (n = 3 mice). Chemokine expression was evaluated by real-time PCR. B: Chemokine levels in the splenic and pancreatic extracts of 6- to 8-week-old Bcl3+/+ and Bcl3−/− NOD mice (n = 3 mice) were tested by immunoblotting and quantified by desitometry using β-actin as a control. C and D: CD4+ T-cells (C) or splenocyte (D) were isolated from 6- to 8-week-old Bcl3+/+ and Bcl3−/− mice and stimulated with plate-bound anti-CD3 (2 μg/ml) for 4 and 16 h (C) or insulin (50 μg/ml) for 16 h (D). Chemokine expression was evaluated by real-time RT-PCR. Data presented are representative of two independent experiments. U, arbitrary unit. *P < 0.01.