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. 2010 Aug 3;59(10):2418–2425. doi: 10.2337/db10-0449

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© 2010 by the American Diabetes Association.

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FIG. 5. — Immunoblot for phospho-AMPK, AMPK, and β-tubulin (A) and optical densitometry of the phospho-AMPK/AMPK ratio (B) from hypothalamus of intravenously infused rats with Vehicle (Veh-IV, n = 8) or OLZ (OLZ-IV, n = 10). Control samples from insulin-induced hypoglycemia (Positive; Pos) or ad libitum–fed (Negative; Neg) rats were also included. C: Relative gene expression (means ± SE) in the hypothalamus of rats intracerebroventricularly infused with OLZ (OLZ-ICV, n = 8) or vehicle (n = 10) following the hyperinsulinemic-euglycemic clamp. *Statistics denote comparison to vehicle group; t test, P < 0.05.