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. 2010 Jul 9;59(10):2360–2365. doi: 10.2337/db10-0088

FIG. 2.

FIG. 2.

A: Response of the insulin sensor to indicated amounts of human insulin. Experiments were performed with a sensor mixture containing 20 nmol/l fluorescein-labeled antibody and 25 nmol/l of Cy5-labeled antibody. Black and gray symbols correspond to experiments performed with sensors using 8E2/3A6 and E6E5/D4B8 antibody pairs, respectively. The average and SD of three measurements are shown. B: Response of the human C-peptide sensor to indicated amounts of C-peptide. Experiments were performed with a sensor mixture containing 20 nmol/l fluorescein-labeled antibody and 25 nmol/l of Cy5-labeled antibody. The average and SD of three measurements are shown. FRET signals were read after a minimum of 20 min of incubation after adding sample to the sensor mix.