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. 2010 Jul 9;59(10):2390–2399. doi: 10.2337/db09-0851

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© 2010 by the American Diabetes Association.

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FIG. 1. — Assessment of transgene expression and activity in S6KCA^RIP and wild-type (WT) mice. A: Staining for HA-tag (red) and insulin (green) of pancreatic sections from 3-month-old wild-type and S6KCA^RIP mice. B: Immunoblotting for HA-tag in islet lysates from 3-month-old wild-type and transgenic mice. C: S6 kinase activity was assessed by immunoblotting for phospho-S6 protein using islets from wild-type and S6KCA^RIP animals cultured overnight in 2 mmol/l glucose, 2% serum. Immunoblotting for actin was used as loading control. The figures are representative blots of at least three independent experiments, all in duplicate (n ≥6). Scale bars represent 25 μm. (A high-quality digital representation of this figure is available in the online issue.)