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. 2010 Jul 9;59(10):2390–2399. doi: 10.2337/db09-0851

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© 2010 by the American Diabetes Association.

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FIG. 7. — Immunoblotting for IRS/Akt signaling components in wild-type (WT) and S6KCA^RIP mice. A: Immunofluorescence staining using anti-phospho-GSK3 (red) and insulin (green) in pancreatic sections from wild-type and S6KCA^RIP mice. B: Immunoblotting and quantitation for different components of IRS/Akt signaling in islet lysates from wild-type and S6KCA^RIP mice. Scanning densitometry of protein bands and quantitation is presented next to the respective immunoblot (n ≥4). Immunoblotting for actin or tubulin were used as loading control. Quantitation of the data are presented as percentage of the control (n = 4). C: Assessment of phosphorylation of Akt, GSK3β, and 4E-BP1 in islet from wild-type and S6KCA^RIP mice after stimulation with IGF1 (100 nmol/l) for 15 min (n ≥4). Data are presented as mean ± SE (*P < 0.05). Scale bars represent 25 μm. (A high-quality digital representation of this figure is available in the online issue.)