FIG. 6.

GRK2^+/− mice display protection against diet-induced obesity. Wt (open symbols, solid lines) and GRK2^+/− (filled symbols, dotted lines) male mice were fed a CD or HFD for 12 weeks. A: Body weight evolution and (B) percentage of change in body weight during the 12 weeks of treatment. C: Daily food intake on CD or HFD. D: Paraffin-embedded sections of epididymal fat pads from Wt and GRK2^+/− male mice fed on CD or HFD and stained with hematoxylin and eosin (magnification ×10; scale bar 50 μm for all pictures). Relative adipocyte size was calculated as detailed in research design and methods. Data are means ± SEM of 10–20 animals per group. E: GTTs (circles, HFD; squares, CD) and (F) ITTs were performed as in Fig. 4A and C, respectively. *P < 0.01. G: Lysates from Wt and GRK2^+/− male mice fed CD or HFD for 12 weeks treated or not with insulin were processed as in Fig. 4D. Representative immunoblots of four independent experiments are shown. See supplementary Fig. S2 and supplementary Table 1 for detailed quantification of these data. (A high-quality color representation of this figure is available in the online issue.)