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Hepatocyte culture volumes and cell number according to surface area (plate type)
| Plate type | Coating volume (μL) | Cell number/well | Medium volume (μL) |
|---|---|---|---|
| 96 well | 50 | 12,000 | 100 |
| 24 well | 250 | 40,000 | 500 |
| 12 well | 500 | 100,000 | 1,000 |
| 6 well | 1,000 | 330,000 | 2,000 |
Collagen volume (plate pre-coating), cell density, and tissue culture medium volume for plating of freshly isolated mouse hepatocytes
