Table 1.
Load, transport and effects of ICAM-1-targeted nanocarriers for lysosomal delivery in cell culture.
| Non- loaded | ASM | α-Gal | GAA | |
|---|---|---|---|---|
| Enzyme coating efficiency (%) | N/A | 80 | 77 | 86 |
| Enzyme molecule per NC (50:50 mass) | N/A | 230 | 530 | 278 |
| Anti-ICAM molecule per NC (50:50 mass) | 250 | 135 | 138 | 123 |
|
| ||||
| Binding (NCs per cell) | 165 | 160 | 70 | 80 |
| % Internalization (1 h) | 80 | 70 | 67 | 80 |
| % Lysosomal transport (3 h) | 75 | 70 | 70 | 65 |
| % Lysosomal storage degradation (5 h) | N/A | 98 | 70 | 75 |
| Enhanced degradation (anti-ICAM/enzyme NCs: free enzyme) | N/A | 2 | 2.5 | 3 |