Figure 4.

Effect of the selective TRPV4 agonist, GSK101, on M-1 cell [Ca²⁺]_i levels. A. Typical [Ca²⁺]_i trace from one coverslip (average of 10 cells) showing effect of TRPV4 activation following addition of 10 nM GSK101. The [Ca²⁺]_i response is slightly biphasic reaching a peak within 1–6 minutes. B. Summary of GSK101 affects on [Ca²⁺]_i (n=7). C. Representative time course showing the effect of GSK101 on [Ca²⁺]_i in the presence and absence of extracellular Ca²⁺ (0 Ca²⁺). Note the abrupt increase in [Ca²⁺]_i following re-introduction of Ca²⁺ with prior treatment by GSK101. D. Summary of the affect of GSK101 on the peak change in the F340/F380 ratio in the presence and absence of 0 Ca²⁺ (-Ca²⁺, n = 2) and HC-067047 (100 nM, n = 4). *P<0.05.