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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Cell Calcium. 2011 Dec 26;51(2):131–139. doi: 10.1016/j.ceca.2011.11.011

Figure 6.

Figure 6

Effect of BK antagonist, IbTX, and SK3 antagonist, apamin, on [Ca2+]i response to HYPO in M-1 cells. A. Representative trace showing the effect of HYPO on [Ca2+]i followed by a repeat HYPO stimulation in the presence of IbTX (100 nM). B. Representative tracing showing that IbTX under isotonic control conditions had no affect on [Ca2+]i (arrow), and reversing the order of IbTX on the HYPO response showed the same inhibition by IbTX on HYPO-induced [Ca2+]i changes. C. Typical [Ca2+]i response to HYPO followed by HYPO response in the presence of apamin (300 nM). D. Summary response of resting [Ca2+]i ratio upon addition of IbTX (n = 3), apamin (n = 3), and IbTX + apamin (n = 3) under control, isotonic (ISO), conditions. The inhibitors had no affect on the resting [Ca2+]i levels. E. Summary showing the HYPO-induced changes in [Ca2+]i ratio (Δ), relative to the control HYPO response (100%), in the presence of IbTX (68 ± 6%, n = 6), apamin (72 ± 4%, n = 4), and IbTX + apamin (28 ± 10%, n = 3). Both inhibitors modestly reduced the HYPO-induced increase in [Ca2+]i ratio, but the combined response showed a much strong reduction in [Ca2+]i. *P<0.05.