Fig. 2.

Effect of cisplatin treatment in HTLV-1 infected Hut-102 or Tax-inducible JPX9 cell lines. PARP cleavage after cisplatin treatment was evaluated by Western blot. Protein aliquots corresponding to 4 × 10⁵ of HTLV-1 infected Hut-102 and HTLV-1 negative control MOLT-3 (a) or Tax-inducible JPX9 (b) cells were separated by SDS-PAGE, transferred to PVDF membranes and then immunodecorated with anti-PARP or anti-Tax antibodies. To induce Tax expression, JPX9 cells were grown for 24 h in medium containing 10 µM CdCl₂ before treatment with cisplatin. Histograms represent percentage of cleaved PARP, resulting from densitometric analysis