Fig. 3.

Flow cytometry analyses of GFP-amastigotes after in vivo G418 selection. Flow cytometry of murine lesion derived GFP-amastigotes after: 15 days infection and 7 doses of saline treatment (A); 15 days infection and 7 doses of G418 treatment (B); 30 days infection and 5 doses of saline treatment (C); 30 days infection and 5 doses of G418 treatment (D); 30 days infection and 9 doses of saline treatment (E); 30 days infection and 9 doses of G418 treatment (F); 90 days infection and 3 doses of saline treatment (G); 90 days infection and 3 doses of G418 treatment (H); 90 days infection and 6 doses of saline treatment (I); 90 days infection and 6 doses of G418 treatment (J). G418 and saline were administered as described in Materials and Methods.