Fig. 4.
Infection of murine macrophages with GFP-L. amazonensis. (A) Number of amastigotes per macrophage and % of infected macrophages after infection with GFP-promastigotes. (B) The fluorescence signal of noninfected macrophages (control) and infected macrophages. Fluorescence is reported in arbitrary fluorescence units. (C) Microscopic images of infected macrophages. Fluorescence image (C1); Phase contrast image (C2) and Giemsa stained image (C3). Magnification 400×. (D) Flow cytometry of noninfected macrophages (D1) and infected macrophages (D2).