Table 3.

Coupling energy (ΔG, kcal/mol) calculated from mutant cycles, as shown in Figure 7.

Drug	Tonic block		Use-dependent block
	F1586	Y1593	F1586	Y1593
pHM	0.97	0.49	1.79	0.87
HMM	0.75	0.50	1.60	0.78
bHMM	0.49	0.35	1.53	0.73
pHHMM	0.52	0.50	2.00	1.15