Table 1.
Oligonucleotid primers used for single PCR assays.
| Primer pairs | Sequence (5′-3′) | Target gene | PCR Amplicon (bp) | Pathogen | Reference |
|---|---|---|---|---|---|
| Strep.sp. | AGAGTTTGATCCTGGCTCAG | 16S rRNA | 500 | .Streptococcus sp | Conrads et al., 1997 |
| FW/BW | GTACCGTCACAGTATGAACTTTCC | ||||
| Entero.sp | TAC TGA CAA ACC ATT CAT GAT G | 16S rRNA | 112 | .Enterococcus sp | Ke et al., 1999 |
| FW/BW | AAC TTC GTC ACC AAC GCG AAC | ||||
| Spa2152 | TTTCGTCTGAGGCAATGTTG | 23S rRNA | 718 | S. parauberis | Riffon et al., 2001 |
| Spa2870 | GCTTCATATATCGCTATACT | ||||
| LOX-1 | AAGGGGAAATCGCAAGTGCC | IctO | 870 | S. iniae | Mata et al., 2004 |
| LOX-2 | ATATCTGATTGGGCCGTCTAA | ||||
| PlG-1 | CATAACAATGAGAATCGC | 16S rRNA | 1100 | L. garvieae | Mata et al., 2004 |
| PlG-2 | GCACCCTCGCGGGTTG | ||||
| FW/BW (V1/V2) | V1:5′-TTTGGTGTTTACACTAGACTG-3′ | 16SrRNA | 120 | S.agalactiae | Meiri-Bendek et al., 2002 |
| V2: 5′-TGTGTTAATTACTCTTATGCG-3′ | |||||
| FW/BW (Strd-dyl/Dys-16s-23s-2) | 5′-TGGAACACGTTAGGGTCG-3′ | 16S-23S rDNA | 300 | S.dysgalactiae | Forsman et al.,1997; Hassan et al., 2003 |
| 5′CTTAACTAGAAAAACTCTTGATTATTC-3′ |