Fig. 1.

Confirmation of subcloning. A PCR was performed using pET32a (panel a) and ToxoP30 primers (panel b). The resulting amplicon was separated by electrophoresis on a 1.5% agarose gel. The reaction of confirmation enzyme is shown in panel c. Panel a: lanes 1-2, pET32a without the SAG1 insert (200 bp); lanes 3-4, pET32a containing the SAG1 insert (1157 bp); lane 5, DNA ladder. Panel b: lane 1, SAG1 gene (957 bp); lane 2, DNA ladder. Panel c: lane 1, uncut recombinant pET32a; lane 2, Pst1-digested recombinant pET32a (1237 bp); lane 3, DNA ladder; lane 4, uncut pET32a, Pst1 digested pET32a (without the 1237-bp band)