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Cis-splicing and trans-splicing: There are 4 exons in the initial transcript, which contains both exons and introns. In the cis-splicing phenomenon, the mRNA contains 4 exons and 3 introns. The 3 introns are removed, and the exons are connected. In trans-trans-splicing, a pre-trans-splicing molecule attaches exon X to intron 3. The 5'-splice donor is attached to the 3'-splice acceptor (Source Ref. 52).
