Table 1.
Superoxide Dismutase Activity Assay of Samples (Fasciola spp & liver tissues) and Standard SOD Solutions using RANSOD kit
| Solutions | Uninhibited tube* | Inhibited tubes | Standards (St1-5) tubes |
|---|---|---|---|
| Substrates | 850 (µl) | 850 (µl) | 850 (µl) |
| Phosphate buffer,7.0 | 25 (µl) | 0 | 0 |
| Xanthine oxidase | 125 (ul) | 125 (ul) | 125 (ul) |
| Samples (F. hepatica, F.gigantica, healthy and infected liver) | 0 | 25 (µl) | 0 |
| Standard solutions (Superoxide Dismutase) | 0 | 0 | 25 (µl) 0.2, 0.5, 1.0, 2, 4 (U/mg) |
*
Uninhibited tube or negative control includes all components except SOD or samples