Table 2.

Absorbances measures, percentage inhibition calculated, total activity and specific activity of SOD enzyme in sample solutions (mean of 10 samples for each)

Sample tubes	Absorbance 1	Absorbance2	A2-A1	A2-A1/3	Calculated inhibition percent*	Total activity of SOD(U/ml)**	Specific activity of SOD(U/mgl)***
solutionUn inhibited	0.118	0.204	0.086	0.028	0	0	0
F. hepatica extract	0.135	0.202	0.67	0.022	21.42	0.76	0.58
F. gigantica extract	0.144	0.183	0.039	0.013	53.5	1.58	0.55
extractHealthy liver	0.162	0.174	0.012	0.004	85.7	2.84	0.51
Infected liver extract	0.155	0.178	0.23	0.007	75	2.28	1.43

^* The percent inhibition of the test samples correlates with SOD activity using a SOD standard curve.

^** One unit of SOD inhibits the rate of increase in absorbance at 550 nm by 50% under the conditions of the assay.

^*** Specific activity is total activity of SOD per milligram of total protein.