Figure 3.

Oligodendrocytes associate with multiple axons from different permissive Matrigel lines and engage and extend membranes on DRG neuron processes. DRG-neurons were seeded on the highly restrictive micropatterned coverslips in the presence of FdU to eliminate dividing cells. After 7 DIV, cortically derived OPCs were added to the cell culture. Seven days after coculture, cells were fixed. In panels (A–D), cells were stained to reveal oligodendrocytes with an oligodendroglial specific marker (RIP in green, MBP in blue) and DRG neurons with neurofilament (red). Scale bar is 100 μm. In panels (E) and (F), cells were observed via a JSM-6460LV scanning electron microscope. Scale bars are 100 μm (E) and 10 μm (F).