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Dorsal root ganglia neurons and Schwann cells are restricted to Matrigel micropatterns after 7 days of in vitro culture. DRG-derived cells (neurons and Schwann cells) seeded on the highly restrictive micropatterned coverslips and maintained for 7 days. Cultures were fixed and stained to reveal (A) α-tubulin subunit present in both cultured cells types Schwann cells and DRG neurons (green), (B) axonal neurofilament-H (red), and (C) nuclei (Topro-3; blue). Scale bar is 50 μm.
