Figure 1.

Expression of Glcci1 in mouse organs reveals varying tissue expression and strong upregulation in kidney glomeruli. (A) Using RT-PCR, high expression is seen in testis, brain, and thymus; somewhat lower expression is seen in lymph nodes, spleen, and eye; and weak signals are seen in lung, heart, liver, muscle, uterus, and whole kidney. However, RT-PCR of RNA from isolated glomeruli gives a strong signal compared with that in RNA from the rest of the kidney. (B) Northern blot reveals strong expression of mRNAs of 5.0 and 6.0 kb in the thymus and of about 2.0-kb-size mRNA in testis. The other organs reveal weak signals for both the 2.0- and 6.0-kb mRNAs. (C) Western blotting of protein extracted from isolated glomeruli show a strong single band of about 60 kD and a weak staining of same size protein in the rest of the kidney.