Figure 2.

Kidney GLCCI1 expression is restricted to podocytes and mesangial cells. Paraffin sections of adult mouse kidney cortex were immunostained with the polyclonal anti-human GLCCI1 antibody or rabbit IgG (5 μg/ ml) as control. (A) No immunoreactivity was observed in adjacent specimens immunostained with normal rabbit IgG. (B) Low power magnification reveals immunoreactivity exclusively in glomeruli of the mouse kidney cortex. (C) At higher magnification, immunostaining of the glomerular podocytes and mesangium with strong cytoplasm staining is apparent. Magnifications: ×100 in overviews and ×800 in single glomeruli. (D) Immunogold labeling in normal mouse capillaries shows the majority of gold particles to be located in the podocyte foot processes. Bars, A and B, 100 μm; C, 10 μm; and D, 0.5 μm.