Figure 4.

Dexamethasone induces expression of Glcci1 in mouse podocytes and glomeruli. RT-PCR for Glcci1 and β-actin (internal standard) expression was carried out on total RNA isolated from DEX-treated cultured mouse podocytes and glomeruli isolated from mice treated with DEX. (A) Podocytes were cultured at different DEX concentrations, and in one case with the GR antagonist RU486 for 30 minutes before the addition of DEX. (B) Expression in glomeruli isolated from mice treated with DEX (2 mg/kg) for 12 hours. Histograms show an increase in Glcci1 expression after DEX treatment.