Table 2. Fluorescent probes (the probes sequences have been compared by BLAST to our sequences to check their specificity and determine their mismatches).
Specificity | Probe name | Sequence (5′–3′) | Fluorescent dye | % Formamide | References |
---|---|---|---|---|---|
Archaea | Arch915 | GTGCTCCCCCGCCAATTCCT | Cy3 | 10-20-30 | Stahl and Amann (1991) |
Eubacteria | Eub338 | GCTGCCTCCCGTAGGAGT | Cy3 or Cy5 or ATTO488 | 10-20-30-40 | Amann et al. (1990) |
Alphaproteobacteria | ALF968 | GGTAAGGTTCTGCGCGTT | Cy3 | 10-20-30-40 | Manz et al. (1992) |
Betaproteobacteria | BET42a | GCCTTCCCACTTCGTTT | Cy3 | 10-20-30-40 | Manz et al. (1992) |
Deltaproteobacteria | DELTA495b | AGTTAGCCGGCGCTTCCT | Cy3 | 10-20-30-40 | Loy et al. (2002) |
Gammaproteobacteria | GAM42a | GCCTTCCCACATCGTTT | Cy3 | 10-20-30-40 | Manz et al. (1992) |
Epsilonproteobacteria | EPSY549 | CAGTGATTCCGAGTAACG | Cy3 | 20-30 | Lin et al. (2006) |
Bacteroidetes | CF319 | TGGTCCGTGTCTGAGTAC | ATTO488 | 10-20-30-40 | Manz et al. (1996) |
Gammaproteobacteria R. exoculata cephalothoracic clones | LBl32/130 | TCCTGGCTATCCCCCACTAC | ATTO488 | 10-20-30 | Durand et al. (2010) |