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. 2012 Jan 13;12:15. doi: 10.1186/1471-2407-12-15

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Copyright ©2011 Dufau et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Spatio-temporal response of Capan-2 spheroid to gemcitabine. Analysis of gemcitabine response was done on 5 μm frozen sections of Capan-2 spheroids treated 16 h and 48 h with 4.10^-7M gemcitabine. DNA damage was revealed by immunodetection of phosphorylated of γH2AX, S phase checkpoint was monitored on Capan-2 spheroid expressing the geminin-mAG FUCCi green probe and apoptosis was analyzed by immunodetection of cleaved form of PARP. Images were collected using a X10 objective. The scale bar corresponds to 100 μm. Results shown are representative of the examination of 3 sections from 5 spheroids. Each experiment has been repeated 3 times.