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AGE-HSA induced a time-(A) and dose-(B) dependent increase of RhoA activity. HMVECs were treated with 50 mg/L AGE-HSA for 30, 45, 60, 90, or 120 min, or with 12.5, 25, 50, or 100 mg/L AGE-HSA for 120 min. RhoA activity was detected with a luminescence-based G-LISA™ assay. Activity reached its maximum at 60 min with 50 mg/L AGE-HSA. Culture medium was used as blank control and HSA was used as albumin control. *P < 0.05, **P < 0.01 vs control.
