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. 2012 Feb 15;7(2):e31817. doi: 10.1371/journal.pone.0031817

Figure 2. Target of mutagenesis.

Figure 2

In the CDR-H1 loop mutagenesis and VH-gene incorporation scFv gene was fused to gene 9-protein (g9p) of the filamentous phage. In the CDR-H3 loop mutagenesis scFv was fused in-frame to β-lactamase (TEM-1). EB104 was the mutagenesis primer in the CDR-H1 and EB120 in CDR-H3 mutagenesis, respectively. The primer pairs WO375-B1 and A1-pAK400rev were used in the analysis of transformants of the CDR-H3 mutagenesis. (ScFv) Single-chain variable fragment, (VL) immunoglobulin variable light domain gene, (VH) immunoglobulin variable heavy domain gene, (PelB) signal sequence for periplasmic excretion and (Lac P/O) Lac promoter.