Figure 3. No. of accentuated clicks within male advertisement calls.

Median ± interquartile ranges (n = 10 per treatment) for EE2 exposure concentrations of A) 296 µg/L, 2.96 µg/L and 29.6 ng/L and B) 29.6 ng/L, 2.96 ng/L and 0.296 ng/L. Statistical differences were determined using General Linear Mixed models (GLMM). Significant differences from solvent control (CTRL)+human chorionic gonadotropin (hCG) treatment are marked by asterisks (* p≤0.05; ** p≤0.01; *** p≤0.001).