Figure 1. Typical results from DH and TSPE-UH suspension microarrays detecting select pathogens.

Two 17-plex bead arrays were developed for the detection of B. anthracis (Ba), F. tularensis (Ft), Y. pestis (Yp), C. burnetii (Cb) and an internal control for DNA extraction and microarray detection (Bt). The microarrays were based on (A) direct hybridization (DH), or (B) target specific primer extension combined with universal microarray hybridization (TSPE-UH) assay formats. Both microarrays make use of identical amplification products from a 16-plex asymmetric PCR. Mean fluorescence intensity (MFI) is displayed for the different probes that are given in Table 1.