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. 2012 Feb 15;7(2):e31152. doi: 10.1371/journal.pone.0031152

Figure 4. Stk25 expression is not correlated with augmented Tau phosphorylation in a Tauopathy model or in transfected HeLa cells.

Figure 4

A Lysates from brains of 9.5 month old wild-type or Tg4510 mutant animals were immunostained for Stk25 and β-actin (upper panel). The average Stk25 signal normalized to β-actin from 3 separate samples from each genotype showed no statistically significant difference (lower panel). B Overexpression of Stk25-GFP did not lead to increased phosphorylation of co-transfected Tau in HeLa cells. Antibodies used for Westerns indicated at left: AT8 (phospho Ser202/Thr205 Tau) 5E2 total Tau. Sizes of expected Stk25-GFP and endogenous Stk25 are indicated at right.