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. 2012 Feb 15;7(2):e31152. doi: 10.1371/journal.pone.0031152

Figure 5. The Golgi extension and neuronal polarization phenotypes in dab1 mutant neurons is dependent upon mouse strain background.

Figure 5

The appearance of the Golgi apparatus was examined in 100 µm thick sections of the hippocampus at birth by immunostaining for GRASP65 and Ctip2 to identify pyramidal neurons. A In wild-type animals the Golgi apparatus extends several microns into the presumptive apical dendrite in the hippocampi, but in C57BL/6 dab1−/− mutants it is convoluted near the nucleus. The Golgi appears more elongated in the Balb/c dab1−/− mutant mice. Nucleus to Golgi distances were measured on isolated cells (insets). Arrowheads represent points used for measurements. B The nucleus to Golgi tip distances are greater for wild-type than dab1−/− mutants, and greater for BALB/c versus C57BL/6 dab1−/− mutants (*,** p<0.0001, Student's t test). C The number of multiple axon bearing neurons in dab1−/− mutant neurons is reduced on the BALB/c background as compared to the C57BL/6 background. In both cases, knocking down Stk25 leads to a further reduction in neurons with multiple axons and the development of neurons with no axons (* p<0.001 compared to the respective EV-control samples, ** p = 0.01 compared between EV-control samples, Student's t test) (Bar = 20 µm) Values for C57BL/6 samples have been published previously [34] and are shown here for comparison to BALB/c samples.