Figure 4. Northern analysis of SeMV icDNA infiltrated cotyledon leaves of Cyamopsis tetragonoloba plants.

(a) 0.8% TBE agarose gel (EtBr staining) and northern blot analysis of total RNA extracted from SeMV icDNA agroinfiltrated leaves 6 dpi from three different plants. The negative sense ³²P labelled probe used for hybridization was complementary in sequence to the (+) sgRNA. (b) 0.8% TBE agarose gel analysis (EtBr staining) and northern blot analysis of total RNA extracted from SeMV icDNA agroinfiltrated leaves 6 dpi. The positive sense ³²P labelled probe corresponding in sequence to that of (+) sgRNA was used for hybridization.