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. 2012 Feb 15;7(2):e31288. doi: 10.1371/journal.pone.0031288

Figure 2. RTT103 partially suppresses the the MMS sensitivity of yku70.

Figure 2

WT (KRY193) and yku70 (KRY 172) mutants were transformed with empty vector, KM93 and RTT103. 5 µl of 10-fold serial dilutions of yeast cultures were plated on SC-LEU plates containing MMS and incubated at 30°C for 2–3 days. (2b) Quantification of MMS sensitivity. The MMS sensitivity of yku70 was quantified by plating appropriate dilutions on plates with and without MMS. Sensitivity of WT was set to 1. yku70 is approximately 60 fold sensitive and upon overexpression of RTT103 the sensitivity to MMS is reduced by approximately 8 fold. Quantification was done for three independent cultures and error bars show SD.