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. 2012 Feb 15;7(2):e31288. doi: 10.1371/journal.pone.0031288

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Srividya et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(6a) rat1-1 and rai1 mutants are not sensitive to MMS. WT (KRY105), rtt103 (KRY230) WT RAI1 (KRY631), rai1 (KRY632), WT RAT1 (KRY633) and rat1-1 (KRY634) strains were grown to mid-log phase. 5 µl of 10-fold serial dilutions of yeast cultures were plated on YPD plates containing MMS and incubated at 30°C for 2–3 days. (6b) rai1 mutants are not sensitive to HO endonuclease. WT (KRY622), rtt103 (KRY646), rai1 (KRY624), were induced with galactose for HO endonuclease to produce DSBs. The percentage survival on galactose compared to WT was calculated from three independent experiments and error bars denote SD.