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. 2012 Feb 15;7(2):e31494. doi: 10.1371/journal.pone.0031494

Figure 1. Animal model for H1N1 infection.

Figure 1

Mice were infected with sub-lethal dose of PR8 by intra-tracheal inhalation. A. Influenza-induced weight loss of the infected mice started from 4 dpi, reached to the peak at 10 and 11 dpi, after which, body weight recovered gradually. Body weights of all infected mice were measured daily. Data of individual mouse and an average of each time point were plotted with rhombus and line, respectively. B. mRNA of influenza nucleoprotein (NP) in the lung tissue reached to a maximum at 5 dpi, and decreased by 13 dpi. In contrast, tumor necrosis factor (TNF) expression was the highest around 5–7 dpi. mRNA expression in the lung tissue was investigated by qRT-PCR. Beta-actin was used for normalization.