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. 2012 Feb 15;7(2):e31608. doi: 10.1371/journal.pone.0031608

Figure 1. Transfer of high levels of anti-Gag IgG1 Abs from Lamp/gag DNA-immunized mothers to offspring through transplacental and breastfeeding routes.

Figure 1

Female BALB/c mice were primed and boosted with 50 µg of Lamp (L), gag (G) or Lamp/gag (LG) plasmid DNA and mated one day after the boost. (A) Maternal serum and (B) amniotic fluid were obtained by caesarean section from full-term pregnant mice. (C) Breast-milk samples (1∶20) were collected on day 5 after delivery and (D) fetal serum from 0-, 30-, 60- and 90-d-o offspring were evaluated by ELISA using HIV-1 lysates. The results from 4–6 animals per group were expressed as the mean ± SEM. Schematic diagram of the immunization protocol is shown. *p≤0.05 and ***p<0.001 when compared to gag.