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. 2012 Feb 15;7(2):e31608. doi: 10.1371/journal.pone.0031608

Figure 2. Maternal immunization with the Lamp/gag DNA vaccine decreases anti-Gag IgG1 Ab production in immunized offspring.

Figure 2

Female mice were immunized, and 20 days later, they were boosted with 50 µg Lamp (L), gag (G), or Lamp/gag (LG) plasmid DNA and mated one day after the boost. Offspring were either non-immunized or immunized with 5 µg of the same vaccine DNA at 7- and 25-d-o. Offspring serum samples were collected at (A) 30- and (B) 60-d-o (3–8 animals per group). Anti-Gag IgG1 was determined by ELISA using HIV-1 lysates. Bars represent the mean ± SEM. Schematic diagram of the immunization protocols are shown. *p≤0.05 and ***p<0.001 when compared to the respective control offspring from non-immunized (NI) mothers.