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. 2012 Feb 15;7(2):e31608. doi: 10.1371/journal.pone.0031608

Figure 6. Maternal LAMP/gag immunization does not interfere with long-lasting IFN-γ and B-cell responses in immunized offspring.

Figure 6

Offspring from non-immune (NI) or immune (I) mothers were immunized at 7- and 25-d-o with 5 µg of either Lamp/gag (LG) or gag (G) DNA. Some neonates groups from I or NI mothers received a 3rd boost at 6-months-old. (A) IFN-γ SFCs from 6-month-old offspring after stimulation with MHC H-2d class I- and II-restricted pools of Gag peptides from HIV-1. The figure represents 4–5 assays per group (2–3 animals per group); *p≤0.05 when compared with immune offspring from NI mothers. (B) Serum from offspring was collected when they were 6 months old (6 animals per group), and anti-Gag IgG was evaluated by ELISA using HIV-1 lysates. One group received a boost 10 days before analysis. Bars represent the mean ±SEM. *p≤0.05 when compared with immune offspring from NI mothers. Schematic diagram of the immunization protocols are shown **p<0.01 when compared with gag-immunized offspring.