Figure 3. Immunohistochemistry detects that AA treatment attenuates CCl₄-induced activation of HSC in a dosage-dependent manner in rats.

Activation of HSC was determined by a-SMA+ myofibroblast transition. A. Representative picture from a control rat treated with 20% peanut oil. B. Representative picture from a rat treated with CCl₄. C–E. Representative pictures from CCl₄-treated rats received AA in a dosage-dependent manner. F. Quantitation of a-SMA+ cells. Data represent mean ± SEM for groups of 8 animals. *** p<0.001 versus normal control; ^##p<0.01, ^###p<0.001 versus CCl₄-induced disease rats. Magnification: ×200.