Figure 4. Real-time PCR and Western blot analysis show that AA treatment blocks CCl₄-induced liver fibrosis in a dosage-dependent manner in rats.

A. Real-time PCR for a-SMA, collagen type I and III mRNA expression. B. Western blot analysis for a-SMA, collagen type I and III protein expression. Data represent mean ± SEM for groups of 8 animals. *p<0.05, **p<0.01, *** p<0.001 versus normal control; ^#p<0.05, ^###p<0.001 versus CCl₄-induced disease control.