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. 2012 Feb 7;7(2):e31350. doi: 10.1371/journal.pone.0031350

Figure 8. AA blocks TGF-beta1-induced phosphorylation of Smad2/3, a-SMA, and collagen matrix production by HSC-T6 cells in vitro.

Figure 8

Western blot analysis detects that HSC-T6 cells pretreated with AA (20 uM) for overnight blocks TGF-beta1 (1 ng/ml)-induced Smad2/3 phosphorylation at 30 mins (A) and upregulation of α-SMA and collagen I expression at 24 h (B,C). Data represent mean ± SEM for at least 3 independent experiments. **p<0.01, ***p<0.001 versus medium control; #p<0.05, ##p<0.01 versus TGF-beta1-treated or DMSO-treated cells.