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. 2011 Nov 17;25(3):284–289. doi: 10.1038/ajh.2011.214

Figure 1.

Figure 1

Expression of complement 3 (C3) and the C3a receptor in vascular smooth muscle cells (VSMCs) from spontaneously hypertensive rats (SHR) and Wistar–Kyoto (WKY) rats. Total RNA was extracted from quiescent VSMCs of 3-week-old SHR and WKY rats. Semiquantitative determination of the abundance of (a) C3 and (c) C3a receptor messenger RNA (mRNA) was performed by reverse transcription and PCR analysis. The ratio of each mRNA to 18S ribosomal RNA (rRNA) was evaluated by analysis of amplified PCR products. Data were expressed as mean ± s.e.m. (n = 6 from two analyses). (b) Western blot analysis of expression of C3 protein in cultured VSMCs from SHR and WKY rats without or with interferon-γ (IFN-γ). Quiescent VSMCs from 3-week-old SHR and WKY rats were incubated without (control) or with 10 ng/ml IFN-γ for 20 h. The ratio of abundance of IFN-γ protein to α-tubulin was evaluated by densitometric analysis. Data are means ± s.e.m. (n = 3). *P < 0.05, **P < 0.01 between indicated column.