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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Anal Bioanal Chem. 2012 Jan 5;402(5):1889–1898. doi: 10.1007/s00216-011-5604-0

Figure 4. Assay miniaturization and validation.

Figure 4

(a) The assays were miniaturized to 4 ul final volume in 1536-well format. The dose response for inhibition by ssCAG-21 was similar to that of 384-well format. (b) Scatter plot representations of the DMSO plate test results for the HTRF and AlphaScreen assays. The treatments were used: column 1 was treated with titrations of ssCAG-21 (from 125 to 0.004 nM), column 2-3 and 5-48 with DMSO (total signal) and column 4 with DMSO and lacked MBNL1-His6 (basal signal).