Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Feb 14;3(1):e00243-11. doi: 10.1128/mBio.00243-11

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2012 Costa et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

FIG 1 — Shigella and Salmonella class IB chaperones are functionally interchangeable. S. flexneri strains (wild type, Δspa15 mutant, and the Δspa15 mutant complemented with Shigella Spa15 or Salmonella InvB) and expressing the designated FLAG-tagged Shigella (A) or Salmonella (B) effectors were grown under conditions that induce T3SS. The supernatant proteins were precipitated with TCA, separated by SDS-PAGE, and immunoblotted with anti-FLAG and anti-IcsA antibodies. IcsA is an autotransporter and secreted by a mechanism other than type 3 secretion and serves as a loading control. The blots shown are representative of at least three experiments.