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. 2012 Feb 21;3(1):e00007-12. doi: 10.1128/mBio.00007-12

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Copyright © 2012 Gilbert et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 4 — Analysis of membrane anchoring of Cda2. (A) Total membranes were isolated and either mock treated (1st and 4th lanes) or digested with 1 U of purified PI-PLC (2nd, 3rd, 5th and 6th lanes). Samples were subsequently subjected to ultracentrifugation to separate the membrane (Mem)-bound proteins (1st to 3rd lanes) from soluble proteins in the supernatant (Sup) (4th to 6th lanes). (B) Total membranes were isolated and either mock treated (1st and 3rd lanes) or digested with PI-PLC (2nd and 4th lanes). Samples were subjected to phase partitioning with Triton X-114 into detergent (Det) (1st and 2nd lanes) and aqueous (Aq) (3rd and 4th lanes) phases.